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Publications

2011

Guynet C., Cuevas A., Moncalian G., de la Cruz F. The stb Operon Balances the Requirements for Vegetative Stability and Conjugative Transfer of Plasmid R388. PLoS Genetics 2011. DOI:10.1371/journal.pgen.1002073

Peña, A. Ripoll-Rozada J., Zunzunegui S., Cabezón E., de la Cruz F., Arechaga I. Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems. The Journal of Biological Chemistry. 2011. DOI:10.1074/jbc.M110.208942

Curiao T, Canton R., Garcillán-Barcia M.P., de la Cruz F., Baquero F., Coque T.M. Association of Composite IS26-sul3 Elements with Highly Transmissible IncI1 Plasmids in Extended-Spectrum-ß-Lactamase-Producing Escherichia coli Clones from Humans. Antimicrobial Agents and Chemotherapy. 2011. 2451-2457. DOI:10.1074/jbc.M110.208942

Williams G.J.*, Williams R.S.*, Williams J.S.*, Moncalian G*, Arvai A.S., Limbo O., Guenther G., SilDas S., Hammel M., Russell P. & Tainer J.A. ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair. Nature Structure and Molecular Biology 2011 DOI:10.1038/nsmb2038

Delaye, L., González-Domenech, C.M., Garcillán-Barcia, M.P., Peretó, J., de la Cruz, F., Moya, A. Blueprint for a minimal photoautotrophic cell: Conserved and variable genes in Synechococcus elongatus PCC 7942. BMC Genomics, 2011, 12, 25. DOI:10.1186/1471-2164-12-25

Vecino A.J., de la Arada I, Segura R.L., Goñi F.M., de la Cruz F., Arrondo J.L.R., Alkorta I. Membrane insertion stabilizes the structure of TrwB, The R388 conjugative plasmid coupling protein. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2011. In Press. DOI:10.1016/j.bbamem.2010.12.025

2010

Arechaga, I., Martínez-Costa, O.H. , Ferreras, C., Carrascosa, J.L., Aragón, J.J. Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function. FASEB Journal. 2010, 24, 12, 4960-4968. DOI:10.1096/fj.10-165845

Arechaga, I., Swamy, M. , Abia, D. , Schamel, W.A., Alarcón, B., Valpuesta, J.M. Structural characterization of the TCR complex by electron microscopy. International Inmunology. 2010, 22, 11, 897-903. DOI:10.1093/intimm/dxq443

Matillla I., Alfonso C., Rivas G., Bolt E.L., de la Cruz F., Cabezon E. The conjugative DNA translocase TrwB is a structure-specific DNA-binding protein. J. Biol. Chem. 2010, 285, 23, 17537-17544. DOI:10.1074/jbc.M109.084137

Fernandez-Lopez R., del Campo I, Ruiz R., Lanza V., Vielva L., de la Cruz F. Numbers on the edges: A simplified and scalable method for quantifying the Gene Regulation Function. BioEssays, 2010, 32, 4, 346-355. DOI:10.1002/bies.200900164

Canonne, J., Marino, D., Noë, L.D., Arechaga, I., Pichereaux, C., Rossigno, M., Roby, D., Rivas, S. Detection and functional characterization of a 215 amino acid n-terminal extension in the Xanthomonas type III effector XopD. PLoS ONE. 2010, 5 (12), art. no. e15773. DOI:10.1371/journal.pone.0015773

de Paz H.D., Larrea D., Zunzunegui S., Dehio C., de la Cruz F., Llosa M. Functional dissection of the conjugative coupling protein TrwB. J. Bacteriol. 2010, 192, 11, 2655-2669. DOI:10.1128/JB.01692-09

Mata C., Mirò E., Mirelis B., Garcillán-Barcia M.P., de la Cruz F., Coll P., Navarro F. In vivo transmission of a plasmid coharbouring blaDHA-1 and qnrB genes between Escherichia coli and Serratia marcescens. FEMS Microbiology Letters. 2010, 308, 1, 24-28 DOI: 10.1111/j.1574-6986.2010.01980.x

Smillie C., Garcillán-Barcia M.P., Francia M.V., Rocha E.P.C., de la Cruz F. Mobility of Plasmids. Microbiology and Molecular Biology Reviews. 2010, 74 (3),434-452. DOI: 10.1128/MMBR.00020-10

de la Cruz F., Frost L.S., Meyer R.J., Zechner E.L. Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiology Reviews 2010, 34 (1), 18-40. DOI: 10.1111/j.1574-6976.2009.00195.x

Lucas M., González-Pérez B., Cabezas M., Moncalian G., Rivas G., de la Cruz F. Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site. J. Biol. Chem. 2010, 285 (12), 8918-8926. DOI: 10.1074/jbc.M109.057539

2009

Valverde, A. , Cantón, R. , Garcillán-Barcia, M.P. , Novais, Â. , Galán, J.C., Alvarado A., de la Cruz F., Baquero F., Coque T.M. Spread of blaCTX-M-14 is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain. Antimicrobial Agents and Gene Therapy. 2009, 53, 12, 5204-5212. DOI:10.1128/AAC.01706-08

Pérez-Mendoza D. and de la Cruz F. 2009. Escherichia coli genes affecting recipient ability in plasmid conjugation: are there any?. BMC Genomics, 10, 71. DOI:10.1186/1471-2164-10-71.

Garcillan-Barcia M.P., Francia M.V., de la Cruz F. 2009. The diversity of conjugative relaxases and its application in plasmid classification. FEMS Micobiol Rev. DOI:10.1111/j.1574-6976.2008.00168.x

Moya A, Gil R, Latorre A, Peretó J, Garcillán-Barcia M. P., de la Cruz F. 2009. Toward minimal bacterial cells: evolution vs. design. FEMS Microbiol Rev. 33:225-35. DOI:10.1111/j.1574-6976.2008.00151.x

Varsaki A, Moncalián G, Garcillán-Barcia M.P., Drainas C, de la Cruz F. 2009. Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking-accessory proteins. J Bacteriol. 2008 Dec 29. DOI:10.1128/JB.01342-08

González-Pérez B., Carballeira J.D., Moncalian G., de la Cruz F. 2009 Changing the recognition site of a conjugative relaxase by rational design Biotech. J. DOI:10.1002/biot.20080018

2008

Acharya SN, Many AM, Schroeder AP, Kennedy FM, Savytskyy OP, Grubb JT, Vincent JA, Friedle EA, Celerin M, Maillet DS, Palmerini HJ, Greischar MA, Moncalian G, Williams RS, Tainer JA, Zolan ME. 2008. Coprinus cinereus rad50 Mutants Reveal an Essential Structural Role for Rad50 in Axial Element and Synaptonemal Complex Formation, Homolog Pairing and Meiotic Recombination. Genetics. 180:1889-907. DOI:10.1534/genetics.108.092775

Jozic D, Cárdenes N, Deribe YL, Moncalián G, Hoeller D, Groemping Y, Dikic I, Rittinger K, Bravo J. 2008. Reply to "The binding stoichiometry of CIN85 SH3 domain A and Cbl-b". Nat Struct Mol Biol.15:891-2. DOI:10.1038/nsmb0908-891

Williams R.*, Moncalian G.*, Williams J., Yamada Y., Limbo O., Shin D., Groocock L., Cahill D., Hitomi C., Guenther G. Mre11 Dimers Coordinate DNA End Bridging and Nuclease Processing in Double-Strand-Break Repair. Cell. Volume 135, 1 , 97-109. DOI:10.1016/j.cell.2008.08.017

Arechaga I, Peña A, Zunzunegui S, Del Carmen Fernández-Alonso M, Rivas G, de la Cruz F. ATPase activity and oligomeric state of TrwK, the VirB4 homologue of plasmid R388 Type IV secretion system. J. Bacteriol. 2008, 190(15), 5472-9. DOI:10.1128/JB.00321-08

Garcillán-Barcia MP, de la Cruz F. Why is entry exclusion an essential feature of conjugative plasmids?. Plasmid. 2008, 60(1), 1-18. DOI:10.1016/j.plasmid.2008.03.002

Revilla C, Garcillán-Barcia MP, Fernández-López R, Thomson NR, Sanders M, Cheung M, Thomas CM, de la Cruz F. Different pathways to acquire resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob. Agents Chemother. 2008, 52(4), 1472-80. DOI: 10.1128/AAC.00982-07

2007

Tato I, Matilla I, Arechaga I, Zunzunegui S, de la Cruz F, Cabezon E. The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: Implications for a common assembly mechanism of DNA translocating motors. J Biol Chem. 2007, 282(35), 25569-76. DOI:10.1074/jbc.M703464200

Gonzalez-Perez B, Lucas M, Cooke LA, Vyle JS, de la Cruz F, Moncalián G. Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides. EMBO J. 2007, 26(16), 3847-57. DOI:10.1038/sj.emboj.7601806

Sanchez-Magraner L, Viguera AR, Garcia-Pacios M, Garcillan MP, Arrondo JL, de la Cruz F, Goni FM, Ostolaza H. The calcium-binding C-terminal domain of Escherichia coli alpha -haemolysin is a major determinant in the surface-active properties of the protein. J. Biol. Chem. 2007, 282, 16, 11827-11835. DOI:10.1074/jbc.M700547200

Garcillán-Barcia MP, Jurado P, González-Pérez B, Moncalián G, Fernández LA, de la Cruz F. Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies. Mol Microbiol. 2007. 63(2):404-16. DOI:10.1111/j.1365-2958.2006.05523

2006

Moncalián G, Cardenes N, Deribe YL, Spinola-Amilibia M, Dikic I, Bravo J.. "Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain." J. Biol. Chem. 2006, 281(50), 38845-53. DOI:10.1074/jbc.M606411200

César CE, Machón C, de la Cruz F, Llosa M. "A new domain of conjugative relaxase TrwC responsible for efficient oriT-specific recombination on minimal target sequences". Mol Microbiol. 2006, 62(4), 984-996. DOI:10.1111/j.1365-2958.2006.05437.x

Fernández-López R, Garcillán-Barcia MP, Revilla C, Lázaro M, Vielva L, de la Cruz F. "Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution". FEMS Microbiol. Rev. 2006, 30(6):942-966. DOI:10.1111/j.1574-6976.2006.00042.x

Yim G, de la Cruz F, Spiegelman GB, Davies J. Transcription modulation of S. typhimurium promoters by sub-MIC levels of rifampicin. J. Bacteriol. 2006, 188(22), 7988-7991. DOI:10.1128/JB.00791-06

Perez-Mendoza D, Lucas M, Munoz S, Herrera-Cervera JA, Olivares J, de la Cruz F, Sanjuan J. "The relaxase of the Rhizobium etli symbiotic plasmid shows nic site cis-acting preference". J. Bacteriol. 2006, 188(21):7488-7499. DOI:10.1128/JB.00701-06

Hormaeche I, Segura RL, Vecino AJ, Goni FM, de la Cruz F, Alkorta I. "The transmembrane domain provides nucleotide binding specificity to the bacterial conjugation protein TrwB". FEBS Lett 2006, 580(13):3075-82. DOI:10.1016/j.febslet.2006.04.059

Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Pérez-Luque R, Coll M, de la Cruz F (2006). "Unveiling the Molecular Mechanism of a Conjugative Relaxase: The Structure of TrwC Complexed with a 27-mer DNA Comprising the Recognition Hairpin and the Cleavage Site". J Mol Biol 358(3):857-869. DOI:10.1016/j.jmb.2006.02.018

Cabezón E, de la Cruz F "TrwB: An F1-ATPase-like molecular motor involved in DNA transport during bacterial conjugation". Res. Microbiol. 2006, 157:299–305. DOI:10.1016/j.resmic.2005.12.002

2005

Jozic D, Cardenes N, Deribe YL, Moncalián G, Hoeller D, Groemping Y, Dikic I, Rittinger K, Bravo J. "Cbl promotes clustering of endocytic adaptor proteins". Nat. Struct. Mol. Biol. 2005, 12, 972-979. DOI:10.1038/nsmb1000

de la Cruz F, Davies J (2005).“Industrial revolution and microbial evolution”. In “The influence of cooperative bacteria on animal host biology” (Eds. M.J. McFall-Ngai, B. Henderson, E.G. Ruby), pp. 73-82. Advances in Molecular and Cellular Microbiology, vol. 10., Cambridge University Press, Cambridge USA.

Draper O, César CE, Machón C, de la Cruz F, Llosa M (2005). "Site-specific recombinase and integrase activities of a conjugative relaxase in the recipient cell". Proc Natl Acad Sci USA 102:16385-16390. DOI:10.1073/pnas.0506081102

de Paz HD, Sangari FJ, Bolland S, García-Lobo JM, Dehio C, de la Cruz F, Llosa M. "Functional interactions between Type IV secretion systems involved in DNA transfer and virulence". Microbiology 2005, 151:3505-3516. DOI:10.1099/mic.0.28410-0

Fernández-López R, Machón C, Longshaw CM, Martin S, Molin S, Zechner EL, Espinosa M, Lanka E, de la Cruz F. "Unsaturated fatty acids are inhibitors of bacterial conjugation". Microbiology 2005, 151:3517-3526. DOI:10.1099/mic.0.28216-0

Pérez-Mendoza D, Sepúlveda E, Pando V, Muñoz S, Nogales J, Olivares J, Soto MJ, Herrera-Cervera JA, Romero D, Brom S, Sanjuan J. "Identification of the rctA Gene, Which Is Required for Repression of Conjugative Transfer of Rhizobial Symbiotic Megaplasmids". J Bacteriol. 2005, 187:7341-7350. DOI:10.1128/JB.187.21.7341-7350.2005

Camacho EM, Serna A, Madrid C, Marqués S, Fernández R, de la Cruz F, Juárez A, Casadesús J. "Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica". J Bacteriol. 2005, 187:5691-5699. DOI:10.1128/JB.187.16.5691-5699.2005

Schamel W W A, Aréchaga I, Risueño RM, van Santen HM, Cabezas P, Risco C, Valpuesta JM, Alarcón B. "Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response". J Exp Med. 2005, 202:493–503. DOI:10.1084/jem.20042155

Tato I, Zunzunegui S, de la Cruz F, Cabezón E. "TrwB, the coupling protein involved in DNA transport during bacterial conjugation, is a DNA-dependent ATPase". Proc Natl Acad Sci USA 2005, 102:8156-61. DOI:10.1073/pnas.0503402102

Llosa M, de la Cruz F. "Bacterial conjugation: a potential tool for genomic engineering". Res Microbiol. 2005, 156:1-6. DOI:10.1016/j.resmic.2004.07.008

2004

Moncalián G, de la Cruz F. "DNA binding properties of protein TrwA, a possible structural variant of the Arc repressor superfamily". Biochim Biophys Acta 2004, 1701:15-23. DOI:10.1016/j.bbapap.2004.05.009

Pérez-Mendoza D, Domínguez-Ferreras A, Muñoz S, Soto MJ, Olivares J, Brom S, Girard L, Herrera-Cervera JA, Sanjuan J. "Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d." J Bacteriol. 2004, 186:5753-61. DOI:10.1128/JB.186.17.5753-5761.2004

Gomis-Ruth FX, Sola M, de la Cruz F, Coll M. "Coupling factors in macromolecular type-IV secretion machineries." Curr Pharm Des. 2004, 10:1551-65. [pdf]

Francia MV, Varsaki A, Garcillan-Barcia MP, Latorre A, Drainas C, de la Cruz F. "A classification scheme for mobilization regions of bacterial plasmids." FEMS Microbiol Rev. 2004, 28(1):79-100. DOI:10.1016/j.femsre.2003.09.001

Hormaeche I, Iloro I, Arrondo JL, Goni FM, de la Cruz F, Alkorta I. "Role of the transmembrane domain in the stability of TrwB, an integral protein involved in bacterial conjugation." J Biol Chem. 2004, 279(12):10955-61. DOI:10.1074/jbc.M310422200

Moncalián G, Lengsfeld B, Bhaskara V, Hopfner KP, Karcher A, Alden E, Tainer JA, Paull TT. "The rad50 signature motif: essential to ATP binding and biological function". J Mol Biol. 2004, 335:937-51. DOI:10.1016/j.jmb.2003.11.026

2003

Guasch A, Lucas M, Moncalian G, Cabezas M, Perez-Luque R, Gomis-Ruth FX, de la Cruz F, Coll M. (2003) "Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC." Nat. Struct. Biol. 10(12):1002-10. DOI:10.1038/nsb1017

Seubert A, Hiestand R, de la Cruz F, Dehio C. (2003) "A bacterial conjugation machinery recruited for pathogenesis." Mol Microbiol. 49(5):1253-66. DOI:10.1046/j.1365-2958.2003.03650.x

Llosa M, Zunzunegui S, de la Cruz F. (2003) "Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes." Proc Natl Acad Sci U S A. 100(18):10465-70. DOI:10.1073/pnas.1830264100

Cabezón E, Montgomery MG, Leslie AG, Walker JE. (2003) "The structure of bovine F1-ATPase in complex with its regulatory protein IF1." Nat Struct Biol. 10(9):744-50. DOI:10.1038/nsb966

Aréchaga, I., Miroux, B., Runswick, M. J. & Walker, J. E. (2003). "Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript". FEBS Lett 547, 97-100. DOI:10.1016/S0014-5793(03)00677-X

Jekabsons, M. B., Echtay, K. S., Arechaga, I. & Brand, M. D. (2003). "Molecular properties of purified human uncoupling protein 2 refolded from bacterial inclusion bodies". J Bioenerg Biomembr 35, 409-18. DOI:10.1023/A:1027335713635

Murata, T*., Arechaga, I*., Fearnley, I. M., Kakinuma, Y., Yamato, I. & Walker, J. E. (2003). (*equal contributors) "The membrane domain of the Na+-motive V-ATPase from Enterococcus hirae contains a heptameric rotor". J Biol Chem 278, 21162-7. DOI:10.1074/jbc.M301620200

Varsaki A, Lucas M, Afendra AS, Drainas C, de la Cruz F. (2003) "Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilization." Mol Microbiol. 48(2):481-93. DOI:10.1046/j.1365-2958.2003.03441.x

Arrondo JL, Echabe I, Iloro I, Hernando MA, de la Cruz F, Goñi FM. (2003). "A bacterial TrwC relaxase domain contains a thermally stable alpha-helical core." J Bacteriol. 185(14):4226-32. DOI:10.1128/JB.185.14.4226-4232.2003

Martinez LO, Jacquet S, Esteve JP, Rolland C, Cabezon E, Champagne E, Pineau T, Georgeaud V, Walker JE, Terce F, Collet X, Perret B, Barbaras R. (2003) "Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis." Nature 421:75-9. DOI:10.1038/nature01250

2002

Hormaeche, I., Alkorta, I., Moro, F., Valpuesta J.M., Goñi, F.M. and de la Cruz, F. (2002) “Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation” J Biol Chem. 277:46456-46462. DOI:10.1074/jbc.M207250200

Gomis-Ruth FX, de la Cruz F, Coll M. (2002) "Structure and role of coupling proteins in conjugal DNA transfer." Res Microbiol. 153(4):199-204. [pdf]

Llosa M., F.X. Gomis-Rüth, M. Coll y F. de la Cruz (2002) “Bacterial conjugation: a two-step mechanism for DNA transport” Mol Microbiol. 153:199-204. DOI:10.1046/j.1365-2958.2002.03014.x

Cabezón, E., Butler, P.J., Runswick, M.J., Carbajo, R.J., and Walker, J.E. (2002) "Homologous and heterologous inhibitory effects of ATPase inhibitor proteins on F-ATPases." J Biol Chem. 277: 41334-41341. DOI:10.1074/jbc.M207169200

Gomis-Rüth F.X., G. Moncalián, F. de la Cruz y M. Coll (2002) “Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein: detailed structural features and mapping of the active site cleft” J Biol Chem. 277:7556-7566. DOI:10.1074/jbc.M110462200

Hopfner KP, Craig L, Moncalián G, Zinkel RA, Usui T, Owen BA, Karcher A, Henderson B, Bodmer JL, McMurray CT, Carney JP, Petrini JH, Tainer JA. (2002) "The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair". Nature 418:562-6. DOI:10.1038/nature00922

Machón C., S. Rivas, A. Albert, F.M. Goñi y F. de la Cruz (2002) “TrwD, the hexameric traffic ATPase encoded by plasmid R388 induces membrane destabilization and hemifusion of lipid vesicles.” J Bacteriol. 184:1661-1668. DOI:10.1128/JB.184.6.1661-1668.2002

de la Cruz F., J.M. García-Lobo y J. Davies (2002) “Antibiotic resistance: on how bacterial populations respond to a simple evolutionary force” en “Bacterial Resistance to Antimicrobials: Mechanisms, Genetics, Medical Practice and Public Health” pp. 19-36 (K. Lewis, A. Salyers, H. Taber, y R. Wax, editores) Editorial Marcel Dekker.

Arechaga, I., Butler, P. J. & Walker, J. E. (2002). "Self-assembly of ATP synthase subunit c rings". FEBS Lett 515, 189-93. DOI:10.1016/S0014-5793(02)02447-X

Ledesma, A., de Lacoba, M. G., Arechaga, I. & Rial, E. (2002). "Modeling the transmembrane arrangement of the uncoupling protein UCP1 and topological considerations of the nucleotide-binding site". J Bioenerg Biomembr 34, 473-86. [pdf]

2001

Arechaga, I., Ledesma, A. & Rial, E. (2001). "The mitochondrial uncoupling protein UCP1: a gated pore". IUBMB Life 52, 165-73. DOI:10.1080/15216540152845966

Arechaga, I. & Jones, P. C. (2001). "The rotor in the membrane of the ATP synthase and relatives". FEBS Lett 494, 1-5. DOI:10.1016/S0014-5793(01)02300-6

Arechaga, I. & Jones, P. C. (2001). "Quick guide: ATP synthase". Curr Biol 11, R117. DOI:10.1016/S0960-9822(01)00055-0

Garcillán-Barcia M.P., I. Bernales, M.V. Mendiola, F. de la Cruz (2001) “IS91 rolling-circle transposition” en “Mobile DNA II” (N.L. Craig, R. Craigie, M. Gellert, A. Lambowitz, eds.) ASM Press, Washington.

Cabezón, E., Runswick, M.J., Leslie, A.G., and Walker, J.E. (2001) "The structure of bovine IF(1), the regulatory subunit of mitochondrial F-ATPase". EMBO J. 20: 6990-6996. DOI:10.1093/emboj/20.24.6990

Núñez B., F. de la Cruz (2001) “Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation” Mol Microbiol. 39:1088-1099. DOI:10.1046/j.1365-2958.2001.02308.x

Gomis-Ruth F.X., G. Moncalián, R. Pérez Luque, A. González, E. Cabezón, F. de la Cruz, M. Coll (2001) “The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase” Nature 409:637-641. DOI:10.1038/35054586

Garcillán-Barcia M.P., I. Bernales, M.V. Mendiola, F. de la Cruz (2001) “Single-stranded DNA intermediates in IS91 rolling-circle transposition” Mol Microbiol. 39:494-502. DOI:10.1046/j.1365-2958.2001.02261.x

2000

Krause S., W. Pansegrau, R. Lurz, F. de la Cruz, E. Lanka (2000) “Enzymology of Type IV Macromolecule Secretion Systems: The Conjugative Transfer Regions of Plasmids RP4 and R388 and the cag Pathogenicity Island of Helicobacter pylori Encode Structurally and Functionally Related Nucleoside Triphosphate Hydrolases” J Bacteriol. 182:2761-2770. [Full Text]

de la Cruz F., J. Davies (2000) “Horizontal gene transfer and the origin of species: lessons from bacteria” Trends Microbiol. 8:128-133. DOI:10.1016/S0966-842X(00)01703-0

Cabezón, E., Butler, P.J., Runswick, M.J., and Walker, J.E. (2000) "Modulation of the oligomerization state of the bovine F1-ATPase inhibitor protein, IF1, by pH." J Biol Chem. 275: 25460-25464. DOI:10.1074/jbc.M003859200

Cabezón, E., Arechaga, I., Jonathan, P., Butler, G., and Walker, J.E. (2000). "Dimerization of bovine F1-ATPase by binding the inhibitor protein, IF1" J Biol Chem. 275: 28353-28355. DOI:10.1074/jbc.C000427200

Grandoso G., P. Avila, A. Cayón, M.A. Hernando, M. Llosa, F. de la Cruz (2000) “Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation” J Mol Biol. 295:1163-1172. DOI:10.1006/jmbi.1999.3425

Zechner, E. L., de la Cruz, F., Eisenbrandt, R., Grahn, A. M., Koraimann, G., Lanka, E., Muth, G., Pansegrau, W., Thomas, C. M., Wilkins, B. M., Zatyka, M. (2000). “Conjugative-DNA transfer processes” En "The Horizontal Gene Pool: Bacterial Plasmids and Gene Spread" (Thomas, C. M., ed.), pp. 87-173. Harwood Academic Publishers, London.

Arechaga, I., Miroux, B., Karrasch, S., Huijbregts, R., de Kruijff, B., Runswick, M. J. & Walker, J. E. (2000). "Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F(1)F(o) ATP synthase". FEBS Lett 482, 215-9. DOI:10.1016/S0014-5793(00)02054-8

Stock, D., Gibbons, C., Arechaga, I., Leslie, A. G. & Walker, J. E. (2000). "The rotary mechanism of ATP synthase". Curr Opin Struct Biol 10, 672-9. DOI:10.1016/S0959-440X(00)00147-0

 


Intergenomics Group - Prof. Fernando de la Cruz Laboratory
. Molecular Biology Department. School of Medicine. Universidad de Cantabria & Instituto de Biomedicina y Biotecnología de Cantabria.
Avenida Cardenal Herrera Oria, s/n 39011 Santander (Spain). Tel. +34942201944